The cellular fuel tank. It's the coenzyme that powers every energy and repair reaction in your cells, and the single thing that refills what MOTS-c burns through while it builds you new mitochondria. If you're crashing, tired, or foggy, this is the baseline.
Your starting dose
5units
5 mg
3×/week (Mon / Wed / Fri), or daily if you're running it to fix a MOTS-c crash
Your insulin syringe is marked 0 to 100. That whole line is 1 mL. Draw to the 5 mark.
01
The protocolHow to mix it, how much to draw, and how long a vial lasts.
The numbers
Vial500 mg
Mix with5 mL BAC water (use "No-Sting") → makes 1 mg = 1 unit
Start5 units (5 mg), go slow the first week
Go up by+5 units (5 mg) per dose until it feels comfortable
Sweet spotup to 25 units (25 mg) per shot, the top of the range
Frequency3×/week (Mon / Wed / Fri), or daily if you're running it to fix a MOTS-c crash
WhenAM, fasted, under the skin (belly) or into muscle
CycleRun a total of ~1,000 mg (2 vials), then a 4-week break, repeat
Units made simple: your insulin syringe is marked 0 to 100. That whole line = 1 mL. At this mix, 1 mg = 1 unit, so a 25 mg dose = draw to the 25 mark. No math. Expect a warm tingle as you go up, that's normal, back off if it's too much.
How long one bottle lasts
At 5 mg 5u100 doses per vial (~8 months at 3×/week)
At 25 mg 25u20 doses per vial (~7 weeks at 3×/week)
At 25 mg 25u daily20 doses per vial (~3 weeks)
One 500 mg vial = 500 units total. Because 1 mg = 1 unit here, just divide 500 by your dose to see how many shots you get.
02
What it doesWhat you get, how it does it, and what to know before you run it.
What it does for you
Restores real energyNAD+ is the direct fuel of your ATP engine, the "underpowered, not lazy" story lives here. Coffee borrows energy you don't have; NAD+ refills the tank that makes it.
Reverses the age declineYou lose ~50% of your NAD+ by 40 (and ~80% by 60). Restoring it is topping up the exact thing that fades with age, a big reason energy, recovery, and clarity slip.
Powers your repair crewNAD+ is the required fuel for your sirtuins (SIRT1 to 7), your DNA-repair and cellular-maintenance genes. No NAD+ = the repair crew can't clock in.
Mental clarityYour brain is the hungriest tissue you have. Clearer thinking and less afternoon fog are downstream of the ATP that NAD+ restores.
Builds new mitochondriaThrough SIRT1 → PGC-1α it doesn't just fuel old power plants, it helps grow new ones, which is why it's the perfect partner to MOTS-c.
How it works
NAD+ is a coenzyme your body uses in ~500 reactions, and it's the electron shuttle that lets your mitochondria actually make ATP (energy). It's also the fuel for sirtuins, your DNA-repair and maintenance crew. Levels drop ~50% by age 50 to 60, which is a big part of why energy, recovery, and clarity fade with age. Worse, it's a self-feeding spiral: making new NAD+ costs ATP, so as NAD+ falls, ATP falls, and your body loses the very energy it needs to rebuild its NAD+, you can't fully climb out of a deep dip on coffee and willpower alone. That's why topping it up directly matters. And when MOTS-c signals your body to build a whole new batch of mitochondria, that burns through your NAD+ pool fast, so refilling it isn't optional, it's the "payroll" that funds the repair crew MOTS-c commands.
What to know before you run it
It runs ~500 different reactions. There's almost no energy or repair process in your body that doesn't touch NAD+. That's why refilling it "touches everything at once", energy, focus, recovery, repair.
You've lost half of it by 40. This isn't a slow, gentle fade, levels roughly halve by mid-life and keep dropping. A lot of what people call "just getting older" is really a battery running low.
Coffee vs. NAD+. Caffeine blocks your tired signal and borrows against tomorrow. NAD+ works the opposite way, it restores the machinery that actually makes the energy. One flogs the engine; the other rebuilds it.
Pills barely work, injection is the move. Your gut destroys oral NAD+ (and precursors like NMN/NR are largely wasted too). SubQ is roughly 10× more usable, which is why it's the format that actually moves the needle.
NAD+ is the FIX for the MOTS-c crash. That crash is NAD+ depletion, not ATP, daily subq NAD+ turns ~3 weeks of misery into ~7 days.
It MUST be subcutaneous, not oral, your gut destroys it. Injected NAD+ is far better than NMN/NR pills.
Run it daily (25 mg) during a MOTS-c rebuild; on its own for general energy/anti-aging, 3×/week is plenty.
Pair it first thing AM with CoQ10, the two work the same energy chain.
03
How to run itMixing, injecting, what to eat, and what to take alongside it.
Mixing and injecting
Mix: add the 5 mL of BAC water slowly down the side of the vial. Swirl gently, never shake.
Store: in the fridge after mixing.
Inject: into belly fat (~2 inches from your belly button) or into muscle. Pinch, go straight in, push slow, pushing fast is what makes it sting. Rotate the spot, fresh pin every time.
Normal: the site may burn or go a little red, and you may feel a flush/tingle, it fades. Push slower and it's milder.